Step 4

The video file should be displayed in the same way no matter which method the file was obtained. Be aware that video files are usually have a large file size so it may take a while to open depending on the type of machine running the Capiscope program. To analyse the file use any of the buttons at the top of the program.

Mark Measurement Points is drawn on to the video file with two points. The start point will be placed on the first click and the end point placed on the second click. To see which direction the blood flow is travelling watch the video file with the play () button. It is important that the two points are placed in the same direction as the blood flow as the calculation functions will be incorrect capillary velocity results. You can place as many number of lines on the video file as required. Once all the required amount of points are placed on the video image then press Calculate. If you make any mistakes whilst drawing the lines then just click on the first point of the line and press the Del key on the keyboard.

The first calculation will determine the diameter of the capillary, then press Accept to run the secondary (velocity) calculation. If you only wish to find the diameter of the capillary then press Skip Use.

The velocity calculation will bring up a few windows displaying various results from the video file. One of the most useful is the "Line Scan" which identifies the correlation of the velocity data and the velocity itself. The Capillary Velocity (cbv) is indentified by a black line with the height of the line displaying the velocity. The correlation of the velocity is given by the background colour (blue meaning low correlation and red meaning high correlation). After the video file has finished, a dialog box like the one above will display information about the calculation which has just run.

Note that after the calculation has taken place, the results of the test will be displayed along side the selected line for easy reference. If more than one measurement point was placed onto the line then the calculation will be run again on the next line. Simply repeat the last few stages and more results will be gained from the video file. All information from this can be viewed from the "Capillary Measurement Results" window.

Pressing "Mark functional capillary density" will allow you to draw "freehand" onto the video image. Simply hold down the mouse button and trace on one of the capillaries. When you have finished marking the capillaries press Calculate and a dialog box similar to the right should be displayed. Simply press Accept, Reject Value or Skip Use. If you make any mistakes whilst drawing a line then just click on the first point of the line and press the Del key on the keyboard.