Step 1
Launch CapiScope by clicking on the CapiScope executable icon. When the program has loaded, click on File - Open Cytoscan file... and the following dialog box will be displayed
Step 2
Clicking on "On My Computer or Network Neighborhood" and pressing OK will bring up a standard file dialog box:
Clicking once on any of the header files will result in information about that file. As many of the video files are large in file size this information is useful in determining the correct video to be analysed. Click Open and the video file will be loaded. You can now proceed to step 4. If you are uploading a file using FTP then continue with step 3
Mark Measurement Points is drawn on to the video file with two points. The start point will be placed on the first click and the end point placed on the second click. To see which direction the blood flow is travelling watch the video file with the play ( The first calculation will determine the diameter of the capillary, then press Accept to run the secondary (velocity) calculation. If you only wish to find the diameter of the capillary then press Skip Use.
The velocity calculation will bring up a few windows displaying various results from the video file. One of the most useful is the "Line Scan" which identifies the correlation of the velocity data and the velocity itself. The Capillary Velocity (cbv) is indentified by a black line with the height of the line displaying the velocity and the correlation of the velocity is given by the background colour (blue meaning low correlation and red meaning high correlation). After the video file has finished, a dialog box like the one above will display information about the calculation which has just run.
Video clip of Velocity measurement. Actual video footage is 256 colours and at full frame rate
Step 3
Clicking on "On Cytoscan (using FTP)" and pressing OK will bring up the following dialog box. For FTP file downloading and analysis a "twisted" CAT 5 ethernet cable (direct PC-Cytoscan) is needed. In the dialog box are the following buttons: Settings will bring up information concerning the FTP configuration on the system. Connect will connect the user to the FTP file location site using the settings previously entered. Open will load the selected file displayed in the lower part of the window.
Under "IP address or URL of remote machine" the cytoscan default IP address is 192.168.1.200. This needs to be set to the same IP address as on the Cytoscan system. The PC network settings (in the Network section of Control Panel) need to be set up as described in the Cytoscan and Microsoft documentation. Under "path on remote machine" enter the path under which the video file is stored. Under "Port" the default setting is 21, if described otherwise in the Cytoscan manual use whatever number is given. Under "user name on remote machine" and "password" use whatever details you are given to log on to the system.
Press OK when all the details have been filled.
If the settings have been correctly entered press Connect and folders on the Cytoscan system will be displayed in the left window (similar to the shot on the right). Navigate to the location of the video files in the OPS system and press Open. Notice that when you click on the header file that you can see the header information in the right hand window pane. This information is the same as the header information given during standard file viewing like in step 2.
Step 4
The video file should be displayed in the same way no matter which method the file was obtained. Be aware that video files are usually have a large file size so it may take a while to open depending on the type of machine running the Capiscope program. To analyse the file use any of the buttons at the top of the program.
) button. It is important that the two points are placed in the same direction as the blood flow as the calculation functions will be incorrect capillary velocity results. You can place as many number of lines on the video file as required. Once all the required amount of points are placed on the video image then press Calculate. If you make any mistakes whilst drawing the lines then just click on the first point of the line and press the Del key on the keyboard.
Note that after the calculation has taken place, the results of the test will be displayed along side the selected line for easy reference. If more than one measurement point was placed onto the line then the calculation will be run again on the next line. Simply repeat the last few stages and more results will be gained from the video file. All information from this can be viewed from the "Capillary Measurement Results" window.
Pressing "Mark functional capillary density" will allow you to draw "freehand" onto the video image. Simply hold down the mouse button and trace on one of the capillaries. When you have finished marking the capillaries press Calculate and a dialog box similar to the right should be displayed. Simply press Accept, Reject Value or Skip Use. If you make any mistakes whilst drawing a line then just click on the first point of the line and press the Del key on the keyboard.
Click here to view a sample output of calculation (pdf file - 201K)